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CHAPTER 3 - CONNECTIVE TISSUE
Histology Guide
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MICROSCOPE SLIDE

SLIDE NAMES
MH 024 Mesentery
MH 025 Mesentery
MH 026 Mesentery
TISSUE
Mesentery
(human)
STAINS
Hematoxylin & Eosin (MH 024)
Verhoeff Stain (MH 025)
Azan (MH 026)
FIXATIVE
Zenker's Formaldehyde
IMAGE SIZES
96,060 x 37,797 pixels (MH 024)
13.5 GB
86,338 x 46,724 pixels (MH 025)
15 GB
6.08 GB (MH 024)
4.28 GB (MH 025)
3.94 GB (MH 026)
FILE SIZES
6.08 GB (MH 024)
520 MB (MH 025)
582 MB (MH 026)
OBJECTIVE
40x
PIXEL SIZE
0.3171 µm
SOURCE
Department of Genetics, Cell Biology, and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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University of Minnesota
Department of Genetics, Cell Biology and Development
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Robert L. Sorenson, Ph.D.

Professor Emeritus
University of Minnesota
Department of Genetics, Cell Biology and Development
6-160 Jackson Hall
321 Church St SE
Minneapolis, MN 55455

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MH 024-025-026 Mesentery

Click the thumbnail to show mesentery stained with hematoxylin & eosin.

Hematoxylin & Eosin

Of the fibrous components in connective tissue, hematoxylin & eosin (H&E) stains both collagen and muscle.

Examine the staining of a in this section of mesentery:

  • - the outer wall is composed of collagen fibers stained pink/red
  • - the crenulated structure in the inner wall is composed of elastic fibers that are unstained (or light pink)
  • Reticular Fibers - unstained
  • - the middle region of the wall is composed of smooth muscle stained pink

Although both collagen and muscle are stained pink/red by H&E, they can still be distinguished from each other based on their morphology and location.

H&E Azan Verhoeff
Collagen pink/red blue red
Elastic Fiber colorless colorless black
Reticular Fiber colorless colorless colorless
Muscle pink pink colorless

Click the thumbnail to show mesentery stained with azan.

Azan

Of the fibrous components in connective tissue, azan is used to distinguish collagen from muscle.

Examine the staining of a in this section of mesentery:

  • - the outer wall is composed of collagen fibers stained blue
  • - the crenulated structure in the inner wall is composed of elastic fibers that are unstained (or red)
  • Reticular Fibers - unstained
  • - the middle region of the wall is composed of smooth muscle stained pink/red

Unlike H&E, collagen (blue) and muscle (red) are stained different colors by azan.

H&E Azan Verhoeff
Collagen pink/red blue red
Elastic Fiber colorless colorless black
Reticular Fiber colorless colorless colorless
Muscle pink pink colorless

Click the thumbnail to show mesentery stained with Verhoeff stain.

Verhoeff Stain

Of the fibrous components in connective tissue, Verhoeff stain is used to identify elastic fibers.

Examine the staining of a in this section of mesentery:

  • - the outer wall is composed of collagen fibers stained red
  • - the crenulated structure in the inner wall is composed of elastic fibers stained black. Another band of is found between the layers of muscle and collagen.
  • Reticular Fibers - unstained
  • - the middle region of the wall is composed of smooth muscle that is unstained (or brown)

Unlike H&E and azan, elastic fibers (black) are stained by Verhoeff.

H&E Azan Verhoeff
Collagen pink/red blue red
Elastic Fiber colorless colorless black
Reticular Fiber colorless colorless colorless
Muscle pink pink colorless

Collagen fibers and elastic fibers in connective tissue can be identified by their distinctive appearance by transmission electron microscopy.

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